Flow cytometry analysis service

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "An operator is available for running samples on BD LSRII and FACS Array. The facility can also assist investigators with the setup and running of samples. Investigators are encouraged to learn how to run their experiments on the LSRII and FACS Array at the Tulane site to enable independent usage of the facility allowing for the flexibility to schedule time for analysis during the evenings and weekends. For this purpose, users need to be trained and demonstrate that they know the proper set up, running and shut down procedures. At the LSU site, only trained operators can run the equipment, therefore, arrangements for services need to be scheduled. Among the available analyses: 1. immunophenotyping (cell surface antigens and receptors) 2. intracellular proteins, cytokines, calcium flux, pH and free fatty acid, 3. signal transduction pathways, 4. DNA and RNA content, cell cycle, DNA synthesis, 5. apoptosis (programmed cell death) analysis, based on: - mitochondrial membrane potential - down regulation of BCL-2 - activation of caspases (cysteine proteases) - cleavage of the DNA repair enzyme PARP - DNA fragmentation (TUNEL Assay with anti-BrdU labeling) - translocation of phosphatidylserine from the inner cytoplasmic membrane to the cell surface (Annexin-FITC/PI) The facility will assist with the retrieval and analysis of data and interpretation of results. There are several packages of software available to analyze list data, including Cell Quest, FlowJo, Microsoft Word, Excel, PowerPoint, as well as Photoshop."

3. Contact

   Price, Mary, B.S.

4. Related Resource

   BD LSR II analyzer

5. Related Resource

   BD FACSCalibur

6. Related Resource

   Miltenyi autoMACS separator

7. Related Resource

   BD FACSAria

8. Related Resource

   BD FACSArray

9. Related Resource

   FlowJo

10. Related Resource

    BD CellQuest

11. Service Provided by

    Cell Analysis & Immunology Core Facility (LCRC)

12. Website(s)

    http://lcrc.info/research/cellAnalysis.html#services

13. Related Technique

    Flow cytometry assay

14. ERO_0001053

    Yes