eagle-i Xavier University of LouisianaXavier University of Louisiana
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Flow cytometry analysis service

eagle-i ID

http://xula.eagle-i.net/i/00000135-1bde-1443-77e4-a45080000000

Resource Type

  1. Analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "An operator is available for running samples on BD LSRII and FACS Array. The facility can also assist investigators with the setup and running of samples. Investigators are encouraged to learn how to run their experiments on the LSRII and FACS Array at the Tulane site to enable independent usage of the facility allowing for the flexibility to schedule time for analysis during the evenings and weekends. For this purpose, users need to be trained and demonstrate that they know the proper set up, running and shut down procedures. At the LSU site, only trained operators can run the equipment, therefore, arrangements for services need to be scheduled. Among the available analyses: 1. immunophenotyping (cell surface antigens and receptors) 2. intracellular proteins, cytokines, calcium flux, pH and free fatty acid, 3. signal transduction pathways, 4. DNA and RNA content, cell cycle, DNA synthesis, 5. apoptosis (programmed cell death) analysis, based on: - mitochondrial membrane potential - down regulation of BCL-2 - activation of caspases (cysteine proteases) - cleavage of the DNA repair enzyme PARP - DNA fragmentation (TUNEL Assay with anti-BrdU labeling) - translocation of phosphatidylserine from the inner cytoplasmic membrane to the cell surface (Annexin-FITC/PI) The facility will assist with the retrieval and analysis of data and interpretation of results. There are several packages of software available to analyze list data, including Cell Quest, FlowJo, Microsoft Word, Excel, PowerPoint, as well as Photoshop."
  3. Contact
    Price, Mary, B.S.
  4. Related Resource
    BD LSR II analyzer
  5. Related Resource
    BD FACSCalibur
  6. Related Resource
    Miltenyi autoMACS separator
  7. Related Resource
    BD FACSAria
  8. Related Resource
    BD FACSArray
  9. Related Resource
    FlowJo
  10. Related Resource
    BD CellQuest
  11. Service Provided by
    Cell Analysis & Immunology Core Facility (LCRC)
  12. Website(s)
    http://lcrc.info/research/cellAnalysis.html#services
  13. Related Technique
    Flow cytometry assay
  14. ERO_0001053
    Yes
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    kcespedes
  3. created
    2012-01-26T15:18:00.765-06:00
  4. creator
    tjohnson
  5. modified
    2012-08-02T13:32:27.609-05:00

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