Automated DNA sequencing

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The Genomics Core Facility uses two ABI PRISM 3130XL Genetic Analyzers. These are multi - color fluorescence based DNA analysis system that utilizes the proven technology of capillary electrophoresis with 16 capillaries operating in parallel. This instrument uses a laser to excite fluorescently labeled DNA fragments as they pass the laser window moving down a the capillary array. The fluorescence is detected and signals are sent to a computer and collected by the ABI Data Collection Software. This data is then processed and interpreted by the ABI Sequence Analysis software. The final output is a color profile or a chromatograph which displays peaks and corresponding base calls. Peaks are four different colors, one for each base."

3. Contact

   Sierra, Rosa A., B.S

4. Contact

   Zabaleta, Jovanny, Ph.D.

5. Related Resource

   ABI PRISM 3130xl Genetic Analyzer

6. Service Provided by

   Genomics Core Facility (LCRC)

7. Website(s)

   http://www.medschool.lsuhsc.edu/genetics/genomicscore/Services.html#1

8. Related Technique

   DNA sequencing assay