Quantitative real-time PCR

eagle-i ID

Resource Type

Properties

1. Resource Description

   "The ABI PPRISM 7900HT is an integrated system that detects and quantitates nucleic acid sequences using fluorescent primers and probes (Taqman) or Syber green. This high-throughput real-time PCR system is designed to perform both real-time PCR and post-PCR (end-point) analysis. It is designed to accept user interchangeable 96-well, 384-well and Low Density Array Card sample blocks, allowing for greater flexibility. This instrument also comes with an automation accessory capable of loading and unloading reaction plates. The resulting integrated unit should provide unattended instrument operation for the processing of up to 84 384-well reaction plates. This would allow for unattended processing of at least 5,000 real-time quantitative PCR sample wells per day (24 hour period), and at least 10,000 SNP genotyping samples every hour. Key applications that can be performed on the ABI PRISM 7900HT included Viral quantitation, quantitation of Gene expression, array verification, drug therapy efficacy, DNA damage measurement, quantity control and assay validation, pathogen detection, and genotyping. Whatever the application, qr-PCR is based on the 5’ nuclease assay (TaqMan). Briefly, this assay includes, a pair of un-labeled primers (amplify target gene of interest) and a duel labeled primer (reporter and quencher dye). During PCR amplification, the probe which anneals to a specific complementary sequence between the forward and reverse primer is displaced and gets cleave by the 5’-3’ exonuclease activity of Taq. Once cleaved, the two dyes are not longer in close proximity, which allows emission of the reporter dye."

2. Additional Name

   qr-PCR service

3. Contact

   Sierra, Rosa A., B.S

4. Contact

   Zabaleta, Jovanny, Ph.D.

5. Related Resource

   ABI Prism 7900 HT

6. Service Provided by

   Genomics Core Facility (LCRC)

7. Website(s)

   http://www.medschool.lsuhsc.edu/genetics/genomicscore/Services.html#2

8. Related Technique

   Gene expression assay

9. Related Technique

   Real-time PCR

10. Related Technique

    Quality control

11. Related Technique

    Genotyping assay