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Genotyping service

eagle-i ID


Resource Type

  1. Material analysis service


  1. Resource Description
    "Genome Scan: Applied Biosystems MD10 Linkage Mapping Set. This commercial set is a group of 400 di-nucleotide markers covering the somatic and X chromosomes at an average of 10cM. Panels are arranged by chromosome, facilitating partial genome scans. The electropherogram below is a single panel of the MD10 Linkage Mapping Set composed of 17 markers. Single Nucleotide Extension (SNE): Applied Biosystems SNaPshot kit for single nucleotide extension reactions. This is essentially a "mini-sequencing" reaction using non-fluor primers and the same Big Dye terminators used in fluorescent sequencing. The multi-step process involves 1) PCR of a region containing a known SNP, 2) ExoSap clean-up of excess dNTPs and primers, 3) SNE reaction using an interrogation primer and dye labeled ddNTP's, 4) Sap cleanup and 5) Gel electrophoresis. The result is a single color peak for each specific nucleotide incorporated. The electropherogram below shows six multiplexed SNE reactions."
  2. Contact
    Sierra, Rosa A., B.S
  3. Contact
    Zabaleta, Jovanny, Ph.D.
  4. Service Provided by
    Genomics Core Facility (LCRC)
  5. Website(s)
  6. Related Technique
    Genotyping assay
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016