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Genotyping service
eagle-i ID
http://xula.eagle-i.net/i/00000135-20d4-ae0d-77e4-a45080000000
Resource Type
Properties
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Resource Description
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"Genome Scan: Applied Biosystems MD10 Linkage Mapping Set. This commercial set is a group of 400 di-nucleotide markers covering the somatic and X chromosomes at an average of 10cM. Panels are arranged by chromosome, facilitating partial genome scans. The electropherogram below is a single panel of the MD10 Linkage Mapping Set composed of 17 markers.
Single Nucleotide Extension (SNE): Applied Biosystems SNaPshot kit for single nucleotide extension reactions. This is essentially a "mini-sequencing" reaction using non-fluor primers and the same Big Dye terminators used in fluorescent sequencing. The multi-step process involves 1) PCR of a region containing a known SNP, 2) ExoSap clean-up of excess dNTPs and primers, 3) SNE reaction using an interrogation primer and dye labeled ddNTP's, 4) Sap cleanup and 5) Gel electrophoresis.
The result is a single color peak for each specific nucleotide incorporated. The electropherogram below shows six multiplexed SNE reactions."
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Contact
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Sierra, Rosa A., B.S
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Contact
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Zabaleta, Jovanny, Ph.D.
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Service Provided by
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Genomics Core Facility (LCRC)
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Website(s)
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http://www.medschool.lsuhsc.edu/genetics/genomicscore/Services.html#4
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Related Technique
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Genotyping assay