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Proteomics Core Facility (LCRC)

Director: Chou, Chau-Wen, Ph.D.


The mission of Proteomics Core Facility is to help researchers advance their biomedical research programs. The Facility is equipped with gel electrophoresis units, image analyzers and digitizers, and robotics for mass spectrometry sample preparation. Currently, a Matrix-Assisted Laser Desorption/Ionization Mass Spectrometer instrument implements protein identification. The various applications include the studies of protein expression profile, posttranslational modifications, and partial sequencing of novel proteins. The staff also consult with researchers about their particular research interests and assist with the development of specific applications and solution to problems.






  • MALDI proteomics protocol ( Protocol )

    Includes instructions for:

    - In-Gel Digestion
    - In-gel Trypsin Digestion
    - C18 ZipTip cleaning up
    - MALDI Spotting


  • Mass spectrometry services ( Material analysis service )


    - LTQ LCMS for 1D band, 1-10
    - LTQ LCMS for 1D band, additional samples
    - LTQ LC-MS for 2D gel spots, 1-10
    - LTQ LC-MS for 2D gel spots, additional samples

  • Sample processing ( Material processing service )


    -Automated in-gel trypsin digest (gel slice subjected to destaining and in gel digestion)-first 10 samples

    - Automated in-gel trypsin digest (gel slice subjected to destaining and in gel digestion)-additional spot

    - ZipTip sample cleanup

    - Gel filtration or Microcon MWCO

    - Sample Extraction


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Last updated: 2012-11-06T08:47:04.446-06:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016