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|  Xavier University of Louisiana |
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| Xavier University of Louisiana |
The mission of Proteomics Core Facility is to help researchers advance their biomedical research programs. The Facility is equipped with gel electrophoresis units, image analyzers and digitizers, and robotics for mass spectrometry sample preparation. Currently, a Matrix-Assisted Laser Desorption/Ionization Mass Spectrometer instrument implements protein identification. The various applications include the studies of protein expression profile, posttranslational modifications, and partial sequencing of novel proteins. The staff also consult with researchers about their particular research interests and assist with the development of specific applications and solution to problems.
For 2D DIGE.
Used for traditional 2D gel electrophoresis.
Used for visible stains and films.
With UV/Vis detector.
Coupled with the Thermo-Fisher LTQ XL Linear Ion Trap mass spectrometer.
For 2D DIGE.
For gel imaging analysis and spot picking.
Coupled with Eksigent nanoLC liquid chromatographer.
For visualization of fluorescence dyes.
Includes instructions for:
- In-Gel Digestion
- In-gel Trypsin Digestion
- C18 ZipTip cleaning up
- MALDI Spotting
Includes:
- LTQ LCMS for 1D band, 1-10
- LTQ LCMS for 1D band, additional samples
- LTQ LC-MS for 2D gel spots, 1-10
- LTQ LC-MS for 2D gel spots, additional samples
Includes:
-Automated in-gel trypsin digest (gel slice subjected to destaining and in gel digestion)-first 10 samples
- Automated in-gel trypsin digest (gel slice subjected to destaining and in gel digestion)-additional spot
- ZipTip sample cleanup
- Gel filtration or Microcon MWCO
- Sample Extraction
For 2D DIGE image analysis and spot processing.
For gel imaging analysis and spot picking.